Composite

Part:BBa_K4601234

Designed by: Doriane Blaise   Group: iGEM23_Evry-Paris-Saclay   (2023-09-23)


LacZalpha expression cassette under the control of the J23110 promoter

This part is an expression cassette of the alpha fragment of the LacZ gene derived from the pUC19 cloning vector (BBa_K2448003) under the control of the BBa_J23110 constitutive promoter.

Usage and Biology

This part expresses constitutively the LacZ gene derived from the pUC19 cloning vector (BBa_K2448003). It can be used as a positive control in various experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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